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Microdeletion Analysis by DNA FISH

Cytogenetics Laboratory : Cytogenetics

Test ID/Workstation :
_/CYTO
Specimen Type:
Amniotic fluid, Blood, Bone Marrow, Fetal, Tissue, Tumor
Tube Type:
Sterile tissue culture flask provided by laboratory; Green top (sodium heparin) tube; Sterile container with tissue culture medium
Collection Volume:
Amniotic fluid: 20.0 mL (minimum 5.0 mL) Blood: 5 mL (minimum 1.0 mL) Bone Marrow: 1.0 mL (minimum 0.5 mL) Tissue: 1.0 mm3
Cause for Rejection:
Clotted, non-sterile, or frozen specimen
Storage:
Ambient
Availability:
Mon-Fri (0700-1600) Sat (0900-1300)
Methodology:
Cultured cells are fixed to slides, hybridized overnight to the appropriate DNA probe(s), washed, and counterstained. 10 cells are analyzed using a fluorescence microscope and the images are captured digitally using image analysis software.
Special Instructions:
Include pertinent medical findings on the requisition with suspected diagnosis and/or indication(s) for the testing. Typically must be ordered with chromosome analysis.
Lab/Phone:
330-543-8483
TAT:
24 hours-14 days
Additional Info:
Interpretation is provided with report Not orderable in Epic
CPT Code:
88271 (each probe), 88273, 88291
Synonyms:
FISH; Fluorescent in situ hybridization
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