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Collection Procedure:

LTD-Urine: Specimen Collection Version 6

Specimen Collection: Urines

Specimens should processed as soon as possible. If the specimen is not

processed within 2 hours, refrigeration for up to 24 hours is acceptable. The use

of the BD C&S Preservative Plus Urine Tube is acceptable to hold the urine up to

24 hours at room temperature provided that the system is used.

1. Clean void

Equipment: Specimen container, gloves, 3 antiseptic wipes, U-Bag

(infants and toddlers)

Site preparation:

For female patients, separate the labia and cleanse the urinary meatus

with one antiseptic wipe at a time. Wipe from front to back. For male

patients, cleanse the urinary meatus with one antiseptic wipe at a time.

After the patient has started urinating, collect a small amount of urine

in the specimen container.

2. Foley catheter

Equipment: Gloves, alcohol swabs, 21-25 gauge needle attached to

5cc sterile syringe, sterile specimen cup.

Site preparation:

Swab the foley catheter tubing puncture port with alcohol swab.

Allow to dry

Allow the urine to collect in the tube beneath the puncture port.

Insert the needle into the puncture port.

Aspirate 5cc of urine and place in the sterile specimen container.

3. Twenty-four hour urine collection

Equipment: Plastic specimen container labeled with patient’s name,

unit number, date, time specimen begins and ends.

Notify lab of any missed specimen samples. Follow Laboratory

Test Directory guidelines for specific instructions.

4. Routine Urinalysis

Equipment: Routine urine specimen container

1. Cleanse the peri-anal area with disinfectant moist towelette or

soap and water

2. Rinse.

3. Apply pediatric U-bag, have patient void in plastic cup, or urinal.

4. Pour the specimen into the urine container to the 12 mL level.

5. Urine for GC or Chlamydia DNA detection- ages 14 and over.

Neisseria gonorrheae CT/NG DNA GeneXpert

Chlamydia CT/NG DNA GeneXpert

NOTE: This test is not used for medical-legal purposes or with prepubertal


Collection: First morning voided urine specimen. Urine must be 20-

50mL, not midstream, collect first void in a leak-proof sterile container.

6. Labeling: Properly label specimens with at least two patient identifiers,

such as name, medical record number, and date of birth


Bowden, V.R., Smith Greenberg, C.(2008). Pediatric Nursing Procedures, 2nd Ed.

Philadelphia: Wolters Kluwer/Lippincott Williams & Wilkins.

Isenberg, H.D. (2004). Clinical Microbiology Procedures Handbook, Volume 2.

Washington, D.C.: The ASM Press.

User Assay Training, Xpert CT/NG GeneXpert, February 2013

Jorgensen James. Manual of Clinical Microbiology 11th Edition, ASM Press 2015

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