Skip to main content Skip to main content
Go to homepage

Conjunctival (Bacterial culture or Chlamydia viral culture of the eye)

Collection Procedure:

 

Conjunctival (Bacterial culture or Chlamydia viral culture of the eye)

Policy Tech: Version 6

 

NOTE: To prevent specimen contamination by microorganisms, use sterile
supplies and aseptic technique to collect specimens.

Equipment:

1. Conjunctiva culture- ESwab, mini-tip Collection kit (green capped).

2. Conjunctiva for GC culture- ESwab, mini-tip Collection kit (green capped).

3. Conjunctiva for Chlamydia (viral) culture-Mini-tipped Culturette (green
capped)

and M4(VIRAL TRANSPORT MEDIA- VTM).

Sterile water or sterile saline, sterile gauze.

Procedure:

A. Site Prep: Clean the skin around eye with sterile saline or sterile water
on sterile gauze before specimen collection.

B. Pass the Mini-tip ESwab over the lower conjunctiva. Avoid the
eyelashes and eyelid margin.

C.

1. Conjunctiva culture Break off the mini-tipped swab into the
transport tube containing 1.0mL liquid media.

2. Conjunctiva cultures for Gonorrhea (GC) Place swab back in
culture container.

3. Conjunctiva cultures for Chlamydia
  a. Elute swab into M4(VIRAL TRANSPORT MEDIA- VTM)
  and mix for 10 seconds

  b. Send M4 (VIRAL TRANSPORT MEDIA- VTM) medium
 on ice to Virology.

D. Labeling: Properly label specimens with at least two identifiers such
as name, medical record number, and date of birth

References:

Bowden, V.R., Smith Greenberg, C.(2008). Pediatric Nursing Procedures, 2nd Ed.

Philadelphia: Wolters Kluwer/Lippincott Williams & Wilkins.

Isenberg, H.D. (2004). Clinical Microbiology Procedures Handbook, Volume 2.

Washington, D.C.: The ASM Press.

Jorgensen, Manual of Clinical Microbiology, 11th Edition. 2015 ASM

Summit Mall Play Area
Answer Key:
Click to expand
There are 10 nurses in the picture.

And we have many more pediatric primary care providers in Northeast Ohio. You can meet some of them here.
Summit Mall Play Area
Answer Key:
Click to expand
The five differences are:
– Phone color
– Coat pocket
– Stethoscope earpiece color
– Stethoscope bell dot
– Clipboard paper color

Need help finding a doctor, choosing a location or getting a general question about Akron Children's answered? Call us or fill out the form and we'll help in any way we can.
Summit Mall Play Area
Answer Key:
Click to expand
The two matching doctors are 9 and 14.

With virtual visits, you can see our pediatric experts from the comfort of home or wherever you are.
Summit Mall Play Area
Answer Key:
Click to expand
The correct path:
The Correct Path
We offer many ways to get pediatric care all over Northeast Ohio. Use this page to find the right kind of care and the most convenient location for you.