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Blood Culture Collection, Venipuncture

Collection Procedure:


Policy Tech: Version 9

Special Notes: To prevent specimen contamination by microorganisms, use sterile supplies and aseptic
technique to collect specimens. Obtain cultures prior to starting antibiotic therapy. Notify the physician if
unable to obtain cultures.

Equipment: venipuncture needle, syringe for specimen, chlorhexidine frepp, tourniquet, gauze, adhesive

Blood Culture Media always consider patient’s weight when choosing appropriate bottles
Green Aerobic Bottle FA+: 1 ml minimum to 10 ml maximum (See Weight Chart)
Orange Anaerobic Bottle FN+: 2 ml minimum to 10 ml maximum (See Weight Chart)

Collection Procedure:



1. Clean venipuncture site with chlorhexidine frepp using back and forth friction scrub for 30 seconds. Allow it to dry for 30 seconds.
2. Do not retouch site; if you must repalpate for vein, the area must be recleaned using above
procedure. Perform venipuncture.
3. When required amount of blood is received, remove needle from skin.
4. Hold pressure to site with gauze and apply adhesive bandage when bleeding stops
5. Remove venipuncture needle from syringe and attach blunt fill needle and transfer device
6. Remove tops of blood culture bottles and swab with chlorhexidine
7. Inject blood specimens into bottles, place specimen into the anaerobic Orange bottle, then the
aerobic Green bottle. If you only have enough blood for one bottle, place the specimen in the
aerobic Green bottle. Be careful, the bottles are pressurized and will aspirate all of the blood from
the syringe unless you control the flow with your thumb and index finger. Optimal blood amounts
are necessary or you will have decreased sensitivity which may delay time to detection of a true
positive blood culture.
8. Label bottle. Indicate whether line draw or peripheral. Label specimens with at least two patient
identifiers, such as name, medical record number, and date of birth
9. Discard needle and syringe into a sharps container
10. Send specimen promptly to CCL. If the specimen is coming from the floors, send the audit trail
with specimen that includes initials of person drawing the specimen, date, time and venipuncture site.



Bowden, V.R., Smith Greenberg, C.(2008). Pediatric Nursing Procedures, 2nd Ed. Philadelphia: Wolters

Kluwer/Lippincott Williams & Wilkins.

Isenberg, H.D. (2004). Clinical Microbiology Procedures Handbook, Volume 2. Washington, D.C.: The ASM


Package Insert BacT/Alert FA+ Ref 410851. 4/2013

Package Insert BacT/Alert FN+ Ref 410852. 4/2013


Additional Info:

Blood culture recommended volume based on patient weight


 Patient weight in Kg

Blood volume to collect

Media type and  inoculation volume




(green FA plus)


(orange FN Plus)

1 to 3  (<6.6 lbs.)


1 ml


3.1 to 6 (6.6-13 lbs)


2 ml


6.1 to 9 (13-20 lbs)

4 ml

4 ml


9.1 to 12 (20-26 lbs)


4 ml

2 ml

12.1 to 20 (26-44 lbs)


4 ml

4 ml

20.1 to 25 (44-55 lbs)


5 ml

5 ml

25.1 to 40 (55-88 lbs)



5 ml

> 40 (>88 lbs)



10 ml

(revised 3-25-15)