Collection Volume:
Skin section approximately 4mm in size
Cause for Rejection:
Skin section too small or to dry to grow, contaminated with bacteria or fungi or not received in liquid.
Methodology:
Tissue culture technique for propagation of cells. Electrophoresis for collagen and procollagen assay.
Special Instructions:
Store skin tissue in HBSS, EMEM, or 0.9% NaCl. Deliver ASAP. When fibroblasts are confluent, flasks are shipped to the University of Washington, Seattle, WA. This test is primarily used to rule out Osteogenesis Imperfecta (OI). Please submit patient history along with specific requisition for growth/storage of fibroblasts and any additional test requested. Available from the Virology lab.
