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Aneuploidy Analysis, Chromosomes 13, 18, 21, X and Y by DNA FISH

Cytogenetics Laboratory : Cytogenetics

Test ID/Workstation :
AFISH CYTO
Specimen Type:
Amniotic fluid
Tube Type:
Sterile tissue culture flask provided by laboratory
Collection Volume:
5.0 mL (minimum 3.0 mL)
Cause for Rejection:
Clotted, non-sterile, or frozen specimen; specimen found not to be amniotic fluid
Storage:
Ambient
Availability:
Mon-Fri (0700-1600) Sat (0900-1300)
Methodology:
Uncultured amniocytes are fixed to slides, hybridized overnight to appropriate DNA probe(s), washed, and counterstained. 100-300 cells are analyzed using a fluorescence microscope. Images are captured digitally using image analysis software.
Special Instructions:
Discard the first 2.0 mL of fluid in order to lessen the possibility of maternal cell contamination. The presence of maternal cells in the fluid may compromise the results. Typically in situ hybridization studies are confirmed by standard cytogenetic studies. Amniotic fluid karyotype requires an additional 10.0 mL of amniotic fluid. Include pertinent medical findings on the requisition with suspected diagnosis and/or indication(s) for testing.
Lab/Phone:
330-543-8483
TAT:
24 hours-5 days
Additional Info:
Interpretation is provided with report
CPT Code:
88271x5, 88275, 88291
Synonyms:
FISH; Fluorescent in situ hybridization; FISH Panel; Trisomy Panel
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