Aneuploidy Analysis, Chromosomes 13, 18, 21, X and Y by DNA FISH
Sterile tissue culture flask provided by laboratory
5.0 mL (minimum 3.0 mL)
Cause for rejection:
Clotted, non-sterile, or frozen specimen; specimen found not to be amniotic fluid
Mon-Fri (0700-1600) Sat (0900-1300)
Uncultured amniocytes are fixed to slides, hybridized overnight to appropriate DNA probe(s), washed, and counterstained. 100-300 cells are analyzed using a fluorescence microscope. Images are captured digitally using image analysis software.
Discard the first 2.0 mL of fluid in order to lessen the possibility of maternal cell contamination. The presence of maternal cells in the fluid may compromise the results. Typically in situ hybridization studies are confirmed by standard cytogenetic studies. Amniotic fluid karyotype requires an additional 10.0 mL of amniotic fluid. Include pertinent medical findings on the requisition with suspected diagnosis and/or indication(s) for testing.
OC Power Word:
24 hours-5 days
Interpretation is provided with report
88271x5, 88275, 88291
FISH; Fluorescent in situ hybridization; FISH Panel; Trisomy