Acid Fast Culture

Infectious Disease Laboratories:Microbiology

Test ID/Workstation: TBC/S SUMMA

Specimen Type: Sputum, BAL, Gastric aspirate, Urine, Tissue, CSF, Blood or Bone Marrow, Stool, Throat swab (ESwab) if unable to obtain sputum

Tube Type: Specimen container, Isolator tube (blood or bone marrow)

Collection Volume: Sputum:5-10 mL
Blood or Bone Marrow: 1.5 mL in Isolator tube
Gastric asp.: 5.0 mL or greater
Urine: First void 25-50 mL
BAL: 1.0 mL or greater

Cause for rejection: Specimen sent in nonsterile container. Sputum specimen consisting of all saliva. Specimens with insufficient volume will be rejected.

Storage: Refrigerated

Availability: Sent to reference lab

Methodology: Fluorescent Antibody Stain for AFB, Culture for AFB

Lab/Phone: 330-543-8412

TAT: AFB stain: 24 hours; AFB culture: 8 weeks

Additional Info: Physician will be notified of positive stains and/or cultures

CPT Code: 87116

Synonyms: Mycobacterial Culture; AFB Culture; TB Culture

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