Spectral Karyotype (SKY) Analysis by DNA FISH

Cytogenetics Laboratory:Cytogenetics

Test ID/Workstation: SKY CYTO

Specimen Type: Blood, Bone Marrow, Lymph Node, Tumor, Amniotic Fluid

Tube Type: Blood, Bone Marrow: Green top (sodium heparin) tube; Lymph Node, Tumor: Sterile tissue culture flask provided by laboratory or sterile container with tissue culture medium

Collection Volume: Aminotic Fluid: 20.0 mL (minimum 5.0 mL); Blood: 5.0 mL (minimum 1.0 mL); Bone Marrow: 1.0 mL (minimum 0.5 mL); Tissue: 1.0 mm3

Cause for rejection: Clotted, non-sterile, or frozen

Storage: Ambient

Availability: Mon-Fri (0700-1600) Sat (0900-1300)

Methodology: Following standard chromosome preparation, cells are fixed to slides, hybridized overnight to appropriate DNA probe(s), washed, and counter-stained. 5-10 metaphases are evaluated using a fluorescence microscope and images are captured digitally using image analysis software.

Special Instructions: Include pertinent medical findings on the requisition with suspected diagnosis and/or indication(s) for testing.

OC Power Word: -

Lab/Phone: 330-543-8483

TAT: 5-14 days

Additional Info: Interpretation is provided with report

CPT Code: 88271x5, 88273, 88291

Synonyms: SKY; Multi-color chromosome studies

Requisition Form
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