Spectral Karyotype (SKY) Analysis by DNA FISH
Blood, Bone Marrow, Lymph Node, Tumor, Amniotic Fluid
Blood, Bone Marrow: Green top (sodium heparin) tube; Lymph Node, Tumor: Sterile tissue culture flask provided by laboratory or sterile container with tissue culture medium
Aminotic Fluid: 20.0 mL (minimum 5.0 mL); Blood: 5.0 mL (minimum 1.0 mL); Bone Marrow: 1.0 mL (minimum 0.5 mL); Tissue: 1.0 mm3
Cause for rejection:
Clotted, non-sterile, or frozen
Mon-Fri (0700-1600) Sat (0900-1300)
Following standard chromosome preparation, cells are fixed to slides, hybridized overnight to appropriate DNA probe(s), washed, and counter-stained. 5-10 metaphases are evaluated using a fluorescence microscope and images are captured digitally using image analysis software.
Include pertinent medical findings on the requisition with suspected diagnosis and/or indication(s) for testing.
OC Power Word:
Interpretation is provided with report
88271x5, 88273, 88291
SKY; Multi-color chromosome studies