Microdeletion Analysis by DNA FISH
Amniotic fluid, Blood, Bone Marrow, Fetal, Tissue, Tumor
Sterile tissue culture flask provided by laboratory; Green top (sodium heparin) tube; Sterile container with tissue culture medium
Aminotic fluid: 20.0 mL (minimum 5.0 mL) Blood: 5 mL (minimum 1.0 mL)
Bone Marrow: 1.0 mL (minimum 0.5 mL) Tissue: 1.0 mm3
Cause for rejection:
Clotted, non-sterile, or frozen specimen
Mon-Fri (0700-1600) Sat (0900-1300)
Cultured cells are fixed to slides, hybridized overnight to the appropriate DNA probe(s), washed, and counterstained. 10 cells are analyzed using a fluorescence microscope and the images are captured digitally using image analysis software.
Include pertinent medical findings on the requisition with suspected diagnosis and/or indication(s) for the testing. Typically must be ordered with chromosome analysis.
OC Power Word:
24 hours-14 days
Interpretation is provided with report
88271 (each probe), 88273, 88291
FISH; Fluorescent in situ hybridization