Lab Test Procedure : Urines

1. Clean Void

Equipment
Specimen Container
Gloves
3 Antiseptic wipes
U-Bag (infants and toddlers


Site Preparation
For female patients, separate the labia and cleanse the urinary meatus with one antiseptic wipe at a time. Wipe from front to back. For male patients, cleanse the urinary meatus one antiseptic wipe at a time.

After the patient has started urinating, collect a small amount of urine in the specimen container.

2. Foley Catheter

Equipment
Gloves
Alcohol swabs
21-25 gauge needle attached to 5cc sterile syringe
sterile specimen cup


Site Preparation
Swab the foley catheter tubing puncture port with alcohol swab. Allow to dry. Allow the urine to collect in the tube beneath the puncture port. Insert the needle into the puncture port. Aspirate 5cc of urine and place in the sterile specimen container.

3. Twenty-four Hour Urine Collection

Equipment
Plastic Specimen Container labeled with Patient's name, unit number, date, time collection begins and ends.

TO begin the urine collection,have the patient void. DISCARD THIS URINE SPECIMEN. This is best done with the first morning specimen. Note the time and record on the requisition. From then on collect all the urine that is voided and place in container. If more than one container is needed, please mark the first as #1 of 2 and retain on the floor until collection is comlpete. The final void should be made 24 hours after the first void described earlier. The specimen should be included with the collection. Refrigerate 24 hour urines during collection. Do not use preservatives. Mark each container with patient information, including height and weight, and date and time collection started and finished. Deliver specimens promptly to the laboratory.

4.Routine Urinalysis

Equipment
Routine Urine Specimen Container

Cleanse the perianal area with disinfectant moist towelette or soap and water, rinse. Apply pediatric U-bag, have patient void inplastic cup or urinal. Pour the specimen into the urine container to the 12 mL level.

5. Urine for Latex (Bacterial Antigens)

Equipment
Routine Urine Specimen Container

Apply pediatric U-bag, have patient void in plastic cup or urinal. Pour the specimen into the urine container. A minimum of 5 mL of urine is needed for latex testing. Obtain 10 mL if possible.

6. Urine for GC or Chlamydia DNA detection - ages 13 and over

Equipment
Routine Urine Specimen Container

NOTE This is not used for medical-legal purposes or with pre-pubertal children.

First morining voided urine specimen. Urine must be 20-60 mL, not midstream. collect first void in leak-proof sterile container.


References:
Bowden, V.R., Smith Greenberg, C.(2008). Pediatric Nursing Procedures, 2nd Ed. Philadelphia: Wolters Kluwer/Lippincott Williams & Wilkins.
Isenbery, H.D. (2004). Clinical Microbiology Procedures Handbook, Volume 2. Washington, D.C.: The ASM Press.

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