Lab Test Procedure : Wound Aerobic (Bacterial Culture)

NOTE For superficial, ulcer, nodule, subcutaneous tissue, lesion, abscess, and skin wounds. A gram stain smear will be routinely reported on specimens labeled as "wound" cultures. The gram stain smear results indicate the type of inflammatory response, presence of bacteria, and quality of specimen submitted for culture. A wound order is only for the aerobic bacterial culture. An anaerobic culture must be ordered separately, if needed (See Specimen collection for anaerobes).

Equipment
ESwab collection kit
alcohol pad or chlorhexadine
sterile gauze
betadine solution


Intact skin Cleanse the skin first with chlorhexidine. Let dry for 30 seconds. Remove the swabs from the collection kit package. Swab the surface of the skin. Break off swab at the red score line into the transport tube containing 1.0mL liquid media.

Non-intact skin Cleanse the surface of the wound and surrounding skin with betadine. Remove overlying debris. Remove the swabs from the collection kit package. If exudate is present from an ulcer or nodule, collect it with the sterile swab. If vesicle is present, collect both fluid and cells from the base of the lesion. Break off swab at the red score line into the transport tube containing 1.0mL liquid media.

Needle or syringe Aspiration Cleanse the skin with an iodine scrub for at least one minute. The procedure is performed by a physician. Do not transport syringe with needle attached. Replace needle with plastic cap.

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